CRISPR-Cas9 Mediated Epigenetic Reprogramming of Cardiac Fibroblasts as a Therapeutic Strategy for Post-Infarction Myocardial Remodeling
Keywords:
CRISPR-Cas9, epigenetic reprogramming, cardiac fibroblasts, myocardial infarction, regenerative medicine, fibrosis reversal, iCMsAbstract
Myocardial infarction (MI) triggers extensive fibrosis, driven largely by cardiac fibroblasts (CFs), which compromises cardiac function. Traditional therapies fail to reverse fibrotic remodeling, making regenerative strategies imperative. In recent years, CRISPR-Cas9-based gene editing has emerged as a precision tool for reprogramming somatic cells. By epigenetically reprogramming CFs into induced cardiomyocyte-like cells (iCMs), researchers aim to repair infarcted myocardium. This paper explores the potential of CRISPR-mediated transcriptional modulation to reverse fibrotic phenotypes and initiate regenerative pathways. We contextualize findings, outline a proposed therapeutic pipeline, and review barriers such as delivery, off-target effects, and chromatin accessibility.
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Copyright (c) -1 Giuseppe Antonio (Author)
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